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Braz. oral res. (Online) ; 33: e084, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1019612

RESUMO

Abstract This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6): mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed: healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment: necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.


Assuntos
Animais , Doenças Dentárias/radioterapia , Necrose da Polpa Dentária/radioterapia , Ápice Dentário/efeitos da radiação , Terapia com Luz de Baixa Intensidade/métodos , Cavidade Pulpar/efeitos da radiação , Lasers Semicondutores/uso terapêutico , Apexificação/métodos , Óxidos/uso terapêutico , Células-Tronco , Doenças Dentárias/patologia , Imuno-Histoquímica , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do Tratamento , Ratos Wistar , Silicatos/uso terapêutico , Compostos de Cálcio/uso terapêutico , Compostos de Alumínio/uso terapêutico , Necrose da Polpa Dentária/patologia , Ápice Dentário/patologia , Polpa Dentária/citologia , Cavidade Pulpar/patologia , Combinação de Medicamentos , Sialoproteína de Ligação à Integrina/análise
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